Kathmandu Center for Genomics
and Research Laboratory

Genetics diagnosis at Molecular level
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Training

SHORT TERM TRAINING PROGRANMME Exposure in Molecular Biology Techniques (Level 1)

Duration of Course: 1 day

1. Module I: Extraction of DNA from biological Samples

PCR Amplification of DNA Samples

Electrophoresis of DNA Samples

Eligibility: Students of B.Sc/ B.Tech/ M.Sc/ M.Tech of Medical Lab. Technology, Microbiology, Biotechnology, Biochemistry, Microbial & Food Technology / Allied Sciences (Studying or Passed out).

Fees: 2,500/-

 

 

SHORT TERM TRAINING PROGRANMME Exposure in Molecular Biology Techniques (Level 2)

Duration of Course: 3 days

1. Module I: Extraction of DNA from biological Samples

PCR Amplification of DNA Samples

Electrophoresis of DNA Samples

2.  Module II:

Restriction Digestion of DNA

Ligation of DNA fragments

Eligibility: Students of B.Sc/ B.Tech/ M.Sc/ M.Tech of Medical Lab. Technology, Microbiology, Biotechnology, Biochemistry, Microbial & Food Technology / Allied Sciences (Studying or Passed out).

Fees: 4,000/-

 

SHORT TERM TRAINING PROGRAMME

Hands-on Training in Molecular Biology Techniques (Level 3)

Duration of Course: 1 week

1. Module I: Extraction of DNA/ RNA from biological Samples or diseased Human Subjects

PCR Amplification of DNA/RNA Samples

Electrophoresis of DNA/RNA Samples

2. Module II:

Option I-

Extraction of Plasmid/Genomic DNA

Electrophoresis of Plasmid/Genomic DNA

Restriction Digestion

Ligation of DNA fragments

Option II-

Short term lymphocyte culture

Karyotyping

Diagnosis of Genetic and Chromosomal disorders

Cytogenetic investigation

 

Eligibility: Students of B.Sc/ B.Tech/ M.Sc/ M.Tech of Medical Lab. Technology, Microbiology, Biotechnology, Biochemistry, Microbial & Food Technology / Allied Sciences (Studying or Passed out).

Fees: 8,000/- (An extra 7000/- for coverage of both options of module II)

 

SHORT TERM TRAINING PROGRANMME

Hands-on Training in Molecular Biology Techniques

Duration of Course: 1 month

1.
Module I Handling of Instruments

Laboratory Safety Protocols

Calibration of Instruments

Preparation of Solutions

Functions of chemicals & solutions

2.      Module II:

Extraction of Plasmid/Genomic DNA

Electrophoresis of Plasmid/Genomic DNA

Preparation of Vector DNA and Insert DNA

Restriction Digestion

Ligation of Genes

Competent Cell Preparation

Transformation

Screening of Transformed Cell

3. Module III:

Basics of Bioinformatics-

Tools Literature and Database access

Data Mining – Gene Bank

BLAST & FASTA

Sequence Alignment

 

4.      Module IV: DNA Amplification through PCR

 

5.      Module V: Microbiology

 1: Introduction to microbiology

2: General and safety instructions to work in microbiology lab

 3: Working with autoclave, hot air oven, laminar air flow, microscope, micropipettes, petri plates, etc

4: Study and preparation of culture media

5: sterilization techniques

 6: isolation of microorganisms from clinical and non-clinical samples

·         Pour plate technique

·         Spread plate technique

·         Streaking  technique

 7: identification of the isolated organisms

·         Study of colony characteristics

·         Biochemical tests

·         Staining techniques

 8: Enumeration of the isolated organisms

·         Plate count method

·         Spot plate technique

 9: Preparation and maintenance of pure culture

·         Slant method

·         Subculture

10: Study on isolation and characterization of biofilm forming bacteria

11:Isolation and screening techniques of industrially important microorganisms

12: Antibiotic susceptibility test

                        Immunology

.1: Ouchterlony Double diffusion (ODD)

2: Radial Immu no diffusion (RID)

3: Rocket immune electrophoresis

             Research Methodology

Proposal writing, Research Ethics and  regulations.

Clinical Research

 Introduction to SPSS and its applications:

Eligibility: Students of B.Sc/ B.Tech/ M.Sc/ M.Tech of Medical Lab. Technology, Microbiology, Biotechnology, Biochemistry, Microbial & Food Technology / Allied Sciences (Studying or Passed out).

Fees: 30,000/-

 

Thesis/ Dissertation/ Project Work Support for Students of B.Sc/ B.Tech/ M.Sc/ M.Tech of Medical Lab. Technology, Microbiology, Biotechnology, Biochemistry, Microbial & Food Technology / Allied Sciences.

Fees: Depending on the project work.

 

FOR FURTHER INFORMATION

Please Contact:00977-9849938494